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Table 1 Primers and probes for digital RT-PCR

From: A fast, efficient and high-throughput procedure involving laser microdissection and RT droplet digital PCR for tissue-specific expression profiling of rice roots

Tissue

Position

Gene

Primer name

Oligo sequence

Amplicon size

Probe name

Probe sequence

Tm probe

Fluorophore

Reference

All

LOC_Os01g70380

Serine palmitoyltransferase

Serine_F

TTGCCGTCGATAATCCTGAC

196

pSerine

CCTCGTTCGTTCGTCGCTGACGGC

64.2

HEX

Sato et al. 2013 [15]

Serine_R

GAGGAAGAGGTCGTCAATGG

 
 

TFIIE (LOC_Os10g25770)

Transcription factor 2E

TFIIE_F

TTAGCTGTGTTGGTCATGGG

161

pTFIIE

CGGAAGAGCTGCTTCAGGTCATCGTCG

63

HEX

This work

TFIIE_R

TCCCAGGAGGACATTGTGTA

 
 

EXP’ (LOC_Os07g02340)

Expressed

Exp_F

ATGGGCAGAAGTCGAAGATG

155

pExp

AGCCAGCTTGAGGCCAACAAGAAGGCC

64.9

HEX

This work

Exp_R

TTTGCACTTGGTCTCAGAGG

     

Stele

LOC_Os08g44750

Nodulin-like protein

5NG4_F

GCAGATATGGTGCATCGACA

170

p5NG4

GCCTCCCTCACCCTCGGCGAGAGC

66.4

FAM

Sato et al. 2013 [15]

5NG4_R

CCCAGAGGACGAGGTAGAG

 
 

OsSHR1 (LOC_Os07g39820)

SHR1

SHR1_F

CAAGCCGCCTCCG

79

pSHR1

CGTCCTACAACTCGAGG

70

HEX

Henry et al. 2017 [16]

SHR1_R

TGGACCCGCTCGAC

 

Cortex

LOC_Os10g18820

Plant disease response protein

Dis_F

AAGGGATCCACACTTCAGGT

152

pDis

GCTGCAAGCAGTGGTGAGTGGTCTGTT

63.2

FAM

Sato et al. 2013 [15]

Dis_R

AGTTCTCGAACAGCATCCTC

 
 

LOC_Os06g48950

OsARF19

OsARR19_F

TCCTCAGACTCAGAACACCA

177

pARF19

TGCCTGGGCTGAGCTTGGTTCAGTGG

64.6

FAM

Yamauchi et al. 2019 [17]; Takehisa et al. 2012 [1]

OsARR19_R

GGTTCTGCAGGCATAATTGC

  
 

LOC_Os01g60960

OsLBD1–8

OsLBD1–8_F

CGTCCAAGTCCATATCACCG

198

pLBD1–8

CTTCGCCGCTCCTCCTCCTCCTCC

66.4

FAM

Yamauchi et al. 2019 [17]

OsLBD1–8_R

TTGAGGGAGCTGTAGTCAGT

  

Outer

LOC_Os10g39890

Pollen Ole 1 allergen

Ole_F

TTCTACTTCACCCTGTCCCA

179

pOle

GGACGGTGCCACCTACTGATCGACCGT

65.2

FAM

Sato et al. 2013 [15]

Ole_R

ACAAAGGCCAAACAACACAC

 
 

LOC_Os02g06290

OsHAC4

OsHAC4_F

GGAAGGAGAAGAACCCACAC

188

pHAC4

AGGTGTGCGATCCAGGCTCGCGA

64.5

FAM

Xu et al. 2017 [18]

OsHAC4_R

CTGGCTTTCACTTCGGAGAA

  
 

LOC_Os06g44970

OsPIN2

OsPIN2_F

CCAGAGCGTCATCTGGTACA

80

pPIN2

CCCTCATGCTCTTCCTCTTCG

63.6

FAM

Wang et al. 2018 [19]

OsPIN2_R

GGAACTGCTCGGAGATGAG