A fast, efficient and high-throughput procedure involving laser microdissection and RT droplet digital PCR for tissue-specific expression profiling of rice roots

Table 3 Reagents and materials

Reagent	Chemical formula	Source	Identifier
Absolute ethanol	C₂H₅OH	Honeywell, USA	603–002–00-5
Acetic acid	C₂H₄O₂	VWR, USA	0714–2.5 L
Butanol	CH₃-CH₂-CH₂-CH₂OH	Sigma-Aldrich, USA	B7906-500 ml
Leica-Paraplast XTRA		Leica, Germany	39,603,002
Xylene	C6H4(CH3)2	Sigma-Aldrich, USA	214,736
Nuclease-free water	H₂O	Ambion, USA	M9932
Eosin	C₂₀H₈Br₄O₅	RAL Diagnostics, France	312,710
RNAsecure reagent		Thermo Fisher Scientific, USA	AM7005
RNaseZAP		Sigma-Aldrich, USA	R2020-250 ml
Biopsy foam pads		Simport, Canada	M476–1
Biopsy cassette		Square Mesh Cassette, Orange	70,072-O
Histology cassette		Thermo Scientific, USA	12,677,796
PEN membrane glass slide		Leica, Germany	11,505,190
0.6-mL microcentrifuge tubes		Molecular Bioproducts, USA	3454
ddPCR 96-well PCR plates		Bio-Rad, USA	12,001,925
Pierceable foil heat Seal		Bio-Rad, USA	1,814,040
DG8 cartridges		Bio-Rad, USA	1,804,008
DG8 gasket for ddPCR		Bio-Rad, USA	1,863,009
Droplet generation oil for probes		Bio-Rad, USA	1,863,005
ddPCR droplet reader oil		Bio-Rad, USA	1,863,004
ddRT-PCR kit from Bio-Rad		Bio-Rad, USA	186–4021

ISSN: 2661-8850