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Fig. 5 | BMC Molecular and Cell Biology

Fig. 5

From: Selecting suitable reference genes for qPCR normalization: a comprehensive analysis in MCF-7 breast cancer cell line

Fig. 5

Determination of optimal number of reference genes needed for normalization (geNorm) for both cultures using pairwise Vn/n + 1 analysis. The recommended cutoff is the lowest Vn/n + 1 below the threshold of 0.15. In the present study, both the cultures had V2/3 less than 0.15, indicating addition of a third reference gene would not affect normalization results

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