Selecting suitable reference genes for qPCR normalization: a comprehensive analysis in MCF-7 breast cancer cell line

Table 1 Descriptive Statistics of the Reference Genes Cq (Quantification Cycle) Values

Gene	N ^a		Mean Cq ± S.D ^b		Minimum Cq		Maximum Cq
Gene	A1	A2	A1	A2	A1	A2	A1	A2
*ACTB*	30	45	15.98 ± 0.26	16.11 ± 0.32	15.38	15.53	16.45	16.62
*GAPDH*	30	45	17.13 ± 0.17	17.07 ± 0.39	16.78	16.43	17.52	17.67
*RPL13A*	30	45	21.03 ± 0.29	20.74 ± 0.43	20.71	20.14	21.64	21.70
*PGK1*	30	45	21.08 ± 0.22	21.09 ± 0.61	20.79	20.29	21.76	22.11
*HSPCB*	30	45	20.40 ± 0.29	20.42 ± 0.51	20.02	19.70	20.98	21.77
*RNA28S*	30	45	8.27 ± 0.23	8.35 ± 0.40	7.61	7.83	8.64	9.58
*RNA18S*	30	45	7.93 ± 0.27	8.26 ± 0.49	7.45	7.58	8.47	9.83
*PUM1*	30	45	23.14 ± 0.23	23.08 ± 0.39	22.73	22.35	23.68	24.23
*CCSER2*	30	45	26.58 ± 0.21	26.56 ± 0.39	26.04	26.03	26.95	27.32
*HNRNPL*	30	45	22.73 ± 0.35	22.91 ± 0.56	22.11	21.97	23.52	23.99
*PCBP1*	30	45	22.13 ± 0.22	22.17 ± 0.37	21.78	21.65	22.76	22.94
*SF3A1*	30	45	23.39 ± 0.34	23.51 ± 0.43	22.99	22.78	24.28	24.68

^a N – Total number of Cq values from all triplicates of three lysates. ^b S.D – Standard Deviation

ISSN: 2661-8850