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Table 1 Descriptive Statistics of the Reference Genes Cq (Quantification Cycle) Values

From: Selecting suitable reference genes for qPCR normalization: a comprehensive analysis in MCF-7 breast cancer cell line

Gene N a Mean Cq ± S.D b Minimum Cq Maximum Cq
A1 A2 A1 A2 A1 A2 A1 A2
ACTB 30 45 15.98 ± 0.26 16.11 ± 0.32 15.38 15.53 16.45 16.62
GAPDH 30 45 17.13 ± 0.17 17.07 ± 0.39 16.78 16.43 17.52 17.67
RPL13A 30 45 21.03 ± 0.29 20.74 ± 0.43 20.71 20.14 21.64 21.70
PGK1 30 45 21.08 ± 0.22 21.09 ± 0.61 20.79 20.29 21.76 22.11
HSPCB 30 45 20.40 ± 0.29 20.42 ± 0.51 20.02 19.70 20.98 21.77
RNA28S 30 45 8.27 ± 0.23 8.35 ± 0.40 7.61 7.83 8.64 9.58
RNA18S 30 45 7.93 ± 0.27 8.26 ± 0.49 7.45 7.58 8.47 9.83
PUM1 30 45 23.14 ± 0.23 23.08 ± 0.39 22.73 22.35 23.68 24.23
CCSER2 30 45 26.58 ± 0.21 26.56 ± 0.39 26.04 26.03 26.95 27.32
HNRNPL 30 45 22.73 ± 0.35 22.91 ± 0.56 22.11 21.97 23.52 23.99
PCBP1 30 45 22.13 ± 0.22 22.17 ± 0.37 21.78 21.65 22.76 22.94
SF3A1 30 45 23.39 ± 0.34 23.51 ± 0.43 22.99 22.78 24.28 24.68
  1. a N – Total number of Cq values from all triplicates of three lysates. b S.D – Standard Deviation