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Table 3 Comprehensive analysis of candidate reference gene ranking with dataset from both culture A1 and A2 combined

From: Selecting suitable reference genes for qPCR normalization: a comprehensive analysis in MCF-7 breast cancer cell line

CV%

NormFinder

geNorm

Candidate Gene

CV%

Rank

Candidate Gene

Group S.D

Rank

Candidate Gene

Stability value M

Rank

PCBP1

22.13

1

GAPDH

0.15

1

CCSER2

0.23

1

ACTB

22.28

2

PCBP1

0.16

2

PCBP1

0.23

1

RNA28S

22.74

3

RNA28S

0.21

3

GAPDH

0.25

2

PUM1

22.99

4

CCSER2

0.21

3

ACTB

0.26

3

CCSER2

23.17

5

ACTB

0.21

3

HSPCB

0.28

4

GAPDH

23.62

6

HSPCB

0.24

4

RNA28S

0.29

5

SF3A1

26.49

7

PGK1

0.27

5

PGK1

0.30

6

RNA18S

27.97

8

HNRNPL

0.28

6

HNRNPL

0.31

7

RPL13A

28.11

9

PUM1

0.29

7

SF3A1

0.32

8

HSPCB

28.65

10

RPL13A

0.29

7

PUM1

0.33

9

HNRNPL

35.10

11

RNA18S

0.30

8

RPL13A

0.34

10

PGK1

35.61

12

SF3A1

0.30

8

RNA18S

0.35

11

BestKeeper

Comparative ΔCt

RefFinder

Candidate Gene

S.D

Rank

Candidate Gene

Average S.D

Rank

Candidate Gene

Geomean

Rank

RNA28S

0.25

1

GAPDH

0.30

1

GAPDH

1.97

1

PUM1

0.26

2

PCBP1

0.30

1

PCBP1

2.00

2

ACTB

0.26

3

CCSER2

0.33

2

CCSER2

2.91

3

PCBP1

0.27

4

RNA28S

0.33

2

RNA28S

2.91

3

GAPDH

0.27

4

ACTB

0.33

2

ACTB

4.16

4

CCSER2

0.28

5

HSPCB

0.34

3

PUM1

6.34

5

SF3A1

0.32

6

PGK1

0.36

4

HSPCB

6.51

6

RPL13A

0.33

7

HNRNPL

0.37

5

PGK1

7.84

7

RNA18S

0.34

8

PUM1

0.38

6

HNRNPL

8.85

8

HSPCB

0.35

9

SF3A1

0.38

6

SF3A1

9.32

9

PGK1

0.41

10

RPL13A

0.38

6

RPL13A

9.92

10

HNRNPL

0.43

11

RNA18S

0.38

6

RNA18S

10.93

11