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Table 3 Comprehensive analysis of candidate reference gene ranking with dataset from both culture A1 and A2 combined

From: Selecting suitable reference genes for qPCR normalization: a comprehensive analysis in MCF-7 breast cancer cell line

CV% NormFinder geNorm
Candidate Gene CV% Rank Candidate Gene Group S.D Rank Candidate Gene Stability value M Rank
PCBP1 22.13 1 GAPDH 0.15 1 CCSER2 0.23 1
ACTB 22.28 2 PCBP1 0.16 2 PCBP1 0.23 1
RNA28S 22.74 3 RNA28S 0.21 3 GAPDH 0.25 2
PUM1 22.99 4 CCSER2 0.21 3 ACTB 0.26 3
CCSER2 23.17 5 ACTB 0.21 3 HSPCB 0.28 4
GAPDH 23.62 6 HSPCB 0.24 4 RNA28S 0.29 5
SF3A1 26.49 7 PGK1 0.27 5 PGK1 0.30 6
RNA18S 27.97 8 HNRNPL 0.28 6 HNRNPL 0.31 7
RPL13A 28.11 9 PUM1 0.29 7 SF3A1 0.32 8
HSPCB 28.65 10 RPL13A 0.29 7 PUM1 0.33 9
HNRNPL 35.10 11 RNA18S 0.30 8 RPL13A 0.34 10
PGK1 35.61 12 SF3A1 0.30 8 RNA18S 0.35 11
BestKeeper Comparative ΔCt RefFinder
Candidate Gene S.D Rank Candidate Gene Average S.D Rank Candidate Gene Geomean Rank
RNA28S 0.25 1 GAPDH 0.30 1 GAPDH 1.97 1
PUM1 0.26 2 PCBP1 0.30 1 PCBP1 2.00 2
ACTB 0.26 3 CCSER2 0.33 2 CCSER2 2.91 3
PCBP1 0.27 4 RNA28S 0.33 2 RNA28S 2.91 3
GAPDH 0.27 4 ACTB 0.33 2 ACTB 4.16 4
CCSER2 0.28 5 HSPCB 0.34 3 PUM1 6.34 5
SF3A1 0.32 6 PGK1 0.36 4 HSPCB 6.51 6
RPL13A 0.33 7 HNRNPL 0.37 5 PGK1 7.84 7
RNA18S 0.34 8 PUM1 0.38 6 HNRNPL 8.85 8
HSPCB 0.35 9 SF3A1 0.38 6 SF3A1 9.32 9
PGK1 0.41 10 RPL13A 0.38 6 RPL13A 9.92 10
HNRNPL 0.43 11 RNA18S 0.38 6 RNA18S 10.93 11