Table 4 Pearson’s Correlation between Cq values from culture A1 and A2 from RT-qPCR and log2 (TPM)
Candidate Reference Gene | Pearson Correlation r (Culture A1 vs log2 TPM) | Pearson Correlation r (Culture A2 vs log2 TPM) | Pearson Correlation r (MCF-7 vs log2 TPM) |
|---|---|---|---|
ACTB | 0.215 (P = 0.253) | - 0.028 (P = 0.852) | 0.115 (P = 0.323) |
GAPDH | - 0.316 (P = 0.088) | - 0.315 (P = 0.837) | 0.078 (P = 0.504) |
RPL13A | - 0.018 (P = 0.923) | - 0.132 (P = 0.385) | - 0.071 (P = 0.272) |
PGK1 | - 0.308 (P = 0.097) | 0.002 (P = 0.988) | 0.028 (P = 0.812) |
HSPCB | - 0.053 (P = 0.780) | - 0.013 (P = 0.927) | - 0.004 (P = 0.970) |
PUM1 | 0.229 (P = 0.221) | 0.119 (P = 0.432) | 0.083 (P = 0.478) |
CCSER2 | - 0.015 (P = 0.936) | - 0.049 (P = 0.747) | - 0.029 (P = 0.798) |
HNRNPL | - 0.150 (P = 0.428) | 0.079 (P = 0.601) | 0.069 (P = 0.554) |
PCBP1 | 0.061 (P = 0.745) | - 0.126 (P = 0.407) | - 0.045 (P = 0.702) |
SF3A1 | 0.310 (P = 0.095) | 0.261 (P = 0.082) | 0.132 (P = 0.256) |