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Table 4 Pearson’s Correlation between Cq values from culture A1 and A2 from RT-qPCR and log2 (TPM)

From: Selecting suitable reference genes for qPCR normalization: a comprehensive analysis in MCF-7 breast cancer cell line

Candidate Reference Gene Pearson Correlation r
(Culture A1 vs log2 TPM)
Pearson Correlation r
(Culture A2 vs log2 TPM)
Pearson Correlation r
(MCF-7 vs log2 TPM)
ACTB 0.215 (P = 0.253) - 0.028 (P = 0.852) 0.115 (P = 0.323)
GAPDH - 0.316 (P = 0.088) - 0.315 (P = 0.837) 0.078 (P = 0.504)
RPL13A - 0.018 (P = 0.923) - 0.132 (P = 0.385) - 0.071 (P = 0.272)
PGK1 - 0.308 (P = 0.097) 0.002 (P = 0.988) 0.028 (P = 0.812)
HSPCB - 0.053 (P = 0.780) - 0.013 (P = 0.927) - 0.004 (P = 0.970)
PUM1 0.229 (P = 0.221) 0.119 (P = 0.432) 0.083 (P = 0.478)
CCSER2 - 0.015 (P = 0.936) - 0.049 (P = 0.747) - 0.029 (P = 0.798)
HNRNPL - 0.150 (P = 0.428) 0.079 (P = 0.601) 0.069 (P = 0.554)
PCBP1 0.061 (P = 0.745) - 0.126 (P = 0.407) - 0.045 (P = 0.702)
SF3A1 0.310 (P = 0.095) 0.261 (P = 0.082) 0.132 (P = 0.256)