Fig. 2
From: ESCRT machinery plays a role in microautophagy in yeast
GFP-Pho8 is properly localized on vacuolar membranes in ESCRT mutants. Cells of strains SCU2684 (wild-type; BY4741), SCU5456 (vps27∆), SCU6187 (vps28∆), SCU6188 (vps36∆), and SCU4337 (vps24∆) harboring plasmid pSCU2366 (pGFP-PHO8) pretreated with FM4–64 were treated with rapamycin for 6 h. Fluorescence micrographs are shown. Scale bars, 5 μm