Fig. 6

WRN interacts with the mRNA export receptor NXF1 and with mRNAs. a Whole-cell extracts were treated with RVC or RNase A (see Supplemental Information) and analyzed by EtBr-stained agarose gel after incubation with the Oligo (dT)₂₅ magnetic beads. b Pull down assay with Oligo (dT)₂₅ magnetic beads using whole-cell lysates from HeLa cells. The captured mRNPs were resolved in a polyacrylamide gel and analyzed by Western blot analysis using the indicated antibodies; 1% of the input was loaded in the first lane. CBP80 and tubulin were used as positive and negative controls, respectively. Molecular size markers (in KiloDaltons) are shown. This is a representative pull down of three independent experiments. c Co-IPs assays in HeLa whole-cell extract treated with RVC or Benzonase (see Supplemental Information for details) were performed using the indicated antibodies. The eluted immunocomplexes were analyzed by Western blots using the indicated antibodies. d EtBr-formaldehyde-agarose gels showing rRNA status after the treatment with RVC or Benzonase. This experiment was performed three times with identical results. Molecular size markers (in KiloDaltons) are shown