Fig. 1

Drop in Viability and Metabolic Activity in a Closed Culture. E.coli was grown as mentioned earlier and samples were collected at various time point. Viable cell counts and metabolic activity were determined at different time points. Figures shows the viable cell counts (Panel a) and the metabolic activity (Panel b) of the bacteria at different time points. Panel c and d shows the relative cell numbers at different time points determined using OD₆₀₀ and flow cytometry techniques respectively. All experiments were repeated three times and the graphs represent mean ± SEM