Fig. 2
Electron microscopy of negatively-stained MreB solution before and after induction of polymerization. a MreB monomers (2 μM) in polymerization buffer (5 mM TRIS-HCl, 0.1 mM CaCl2, 0.2 mM ATP, pH 7.5) on EM grid. b-c Filaments formed from the same MreB solution as in A, after induction of polymerization with 10 mM MgCl2. d YFP-MreB monomers (2 μM) in polymerization buffer on EM grid. e-f Filaments formed from the same YFP-MreB solution as in D, after induction of polymerization with 10 mM MgCl2. Scaling is indicated below the black bars