Fig. 6

Co-localization of MreB paralogs in vitro. a MreB paralogs prior to induction of polymerization on a planar membrane. Note that there is no spectral bleed through between the panels, and that there is an area lacking membrane coating that fluctuates between the acquisitions. b Co-polymerization of CFP-Mbl and mCherry-MreBH, each 5 μM, (c) Co-polymerization of CFP-Mbl and YFP-MreB, both 2 μM, (d) Co-polymerization of all three MreB paralogs, each 1 μM, using confocal microscopy, (e) Addition of 2 μM CFP-Mbl (green in overlay) to pre-polymerized YFP-MreB (2 μM, red in overlay), yellow triangles indicate common filamentous structures, green triangles CFP-Mbl structures that assembled independent of preexisting YFP-MreB filaments. f Addition of 2 μM mCherry-MreBH (green in overlay) to preassembled CFP-Mbl (2 μM, red in overlay), yellow triangles in overlay indicate mCherry-MreB filaments assembled at preexisting YFP-MreB filaments, green triangles independent structures. Scalebars 2 μm