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Fig. 3 | BMC Molecular and Cell Biology

Fig. 3

From: Comparison of size distribution and (Pro249-Ser258) epitope exposure in in vitro and in vivo derived Tau fibrils

Fig. 3

Effect of sonication on biochemical and functional analysis of human PHFs. a-c Different dilutions of human AD brain-derived PHFs (sonicated and non-sonicated), were analyzed with an AT8-AT8 (a), PT51-PT51 (b) and PT76/PT76 (c) Tau aggregate selective MSD assays. (D,E) Seeding capacity of PHF Tau seeds (sonicated and non-sonicated) were investigated by adding different amounts of the preparations to K18-FRET cells, in the absence (panel D) or presence (panel E) of transfection reagent. Induction of aggregation was evaluated and expressed as by percentage of FRET. (p < 0.05; p < 0.01; p < 0.001; p < 0.0001, two-way ANOVA analysis)

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