Fig. 3From: Comparison of size distribution and (Pro249-Ser258) epitope exposure in in vitro and in vivo derived Tau fibrilsEffect of sonication on biochemical and functional analysis of human PHFs. a-c Different dilutions of human AD brain-derived PHFs (sonicated and non-sonicated), were analyzed with an AT8-AT8 (a), PT51-PT51 (b) and PT76/PT76 (c) Tau aggregate selective MSD assays. (D,E) Seeding capacity of PHF Tau seeds (sonicated and non-sonicated) were investigated by adding different amounts of the preparations to K18-FRET cells, in the absence (panel D) or presence (panel E) of transfection reagent. Induction of aggregation was evaluated and expressed as by percentage of FRET. (∗p < 0.05; ∗∗p < 0.01; ∗∗∗p < 0.001; ∗∗∗∗p < 0.0001, two-way ANOVA analysis)Back to article page