Fig. 4

Native page analysis of K18 and PHF fibril preparations subjected to sonication. a In vitro aggregated K18 fibrils (sonicated and non-sonicated), non-aggregated K18 and 2N4R (WT) Tau were analyzed by native PAGE as described in materials and methods. Visualization was performed by the presence of Coomassie brilliant blue in the native PAGE sample buffer mix. The left panel shows high MW species in both aggregated K18 samples with a stronger signal for the sonicated sample. The right panel shows the same gel before cassette removal demonstrating presence of large aggregates not entering the gel. b Sonicated and non-sonicated PHF aggregates were analyzed by native PAGE as described in materials and methods. Detection was accomplished with hTau10, AT8, PT51 and PT76 -HRPO labelled antibodies