Fig. 6

Analysis of PHF and P301S brain extract sucrose gradient fractions. Tau fibrils derived from human AD brain (a-c) or from P301S Tau Tg mouse spinal cord (d-f) were separated by sucrose gradient ultracentrifugation. PHF (a) and P301S (d) gradient fractions were analyzed by Western blot under native conditions. Detection performed with PT76 primary HRPO-labelled antibody. (Top: non-sonicated; bottom: sonicated). Fraction 1 has the highest density, fraction 19 has the lowest density. Fraction P is a resuspension of the pellet in PBS. An MSD analysis of PHF (b) and P301S fractions is performed using the indicated aggregate selective assays are represented. Functional analysis of PHF (c) and P301S (f) sucrose gradient fractions was performed by determining seeding capacity in the absence of transfection reagent (P301S) or in the presence of transfection reagent (human PHF). This was evaluated by percentage of FRET-positive cells. A representative profile of at least two independent experiments is shown