Table 1 Primers used for qPCR analysis
Strain/Cell line | Gene/Accession No | Primer sequence | F/R | Product size (bp) |
|---|---|---|---|---|
E coli MG1655 [CP032667] | IS5-like element IS5 family transposase AYG17556.1 [CP032667: 230175–231,191] | 5’GCC GAA CTG TCG CTT GAT GA | F | 217 |
5’ATT TGT CTC AGC CGA TGC CG | R | |||
4 T1 cells [ATCC® CRL-539™] Mus musculus [10090] | BetaActin AC144818.4 [NC000071.6: 73696–73,082] | 5’GAT TAC TGC TCT GGC TCC TAG | F | 147 |
5’GAC TCA TCG TAC TCC TGC TTG | R |