Fig. 6From: Regulatory effect of heat shock transcription factor-1 gene on heat shock proteins and its transcriptional regulation analysis in small abalone Haliotis diversicolorActivity analysis of HdHSF1 gene promoter in HEK293T cells. Based on the length of the seven fragments containing promoter region, the recombinant plasmids were named pGL3-hsf1-r1, pGL3-hsf1-r2, pGL3-hsf1-r3, pGL3-hsf1-r4, pGL3-hsf1-r5, pGL3-hsf1-r6, and pGL3-hsf1-r7. The pRL-TK vector containing the Renilla luciferase gene was transfected as an internal reference to correct the transfection efficiency. The pGL3-Basic plasmid served as a negative control. The different letters on the error bars represent significant differences, p < 0.05. The values are means ± SD of biological replicates (n = 3)Back to article page