Fig. 5

IMFlnc1 regulates CAV-1 expression by sponging miR-199a-5p. a Detection of IMFlnc1 in porcine intramuscular adipocyte by RNA fluorescence in situ hybridization (RNA-FISH). Red represents FISH probes of IMFlnc1. Nuclei are counterstained with DAPI (blue). Scale bar, 50 μm. b The schematic diagram shows the sequences of IMFlnc1 and CAV-1 with miR-199a-5p, inculding wild-type (Wt) and mutant type (Mut). c The binding site of miR-199a-5p at CAV-1 3’UTR (green) are evolutionarily conserved across species. d The correlation analysis of IMFlnc1 expression level and CAV-1 mRNA level in 18 porcine LD muscle tissues. e IMFlnc1 acted as the target of miR-199a-5p. f CAV-1 acted as the target of miR-199a-5p. g IMFlnc1 acted as the sponge of miR-199a-5p. IMFlnc1 increases CAV-1 expression (h) and adipogenesis (i) in a miR-199a-5p dependent manner