Fig. 3

Egr-1 is necessary for the expression of Wnt5a. a Human oral fibroblasts were transfected with Wnt5a promoter luciferase reporter plasmids, treated with 8 μg/ml Arecoline and p65, Smad2, Smad3 or Egr-1 siRNAs for 48 h, then luciferase reporter assays were performed to detect the activity of Wnt5a promoter. b Human oral fibroblasts were treated with 8 μg/ml Arecoline and p65, Smad2, Smad3 or Egr-1 siRNAs for 48 h, then RT-PCR assays were performed to detect the mRNA levels of Wnt5a. c Human oral fibroblasts were treated with 8 μg/ml Arecoline and Egr-1 siRNAs for 24 h, then cell lysates were analyzed by Western blotting using indicated antibodies. * denotes p < 0.05