Fig. 2
From: Role of tumor cell senescence in non-professional phagocytosis and cell-in-cell structure formation
a Immunofluorescence staining of the senescence markers HMGA2 and H3K9me3 in red without and after 6 days treatment with camptothecin (CPT) 120 nM and 200 nM for senescence induction in pancreas carcinoma cells BxPC-3. The cell nuclei were stained with DAPI (blue). Scale bar 10 μm. b Cell nucleus length and width of BxPC-3 cells after 6 days CPT treatment. c C12FDG β-galactosidase activity in untreated and 120 nM treated BxPC-3 cells on day 6. d Activity of the acidic β-galactosidase in pancreas carcinoma cells after CPT treatment on days 5, 6 and 7 using flow cytometry. The graphs represent the mean values from three independent experiments ± standard deviation, p-values: * < 0.05, ** < 0.01, *** < 0.001. e C12FDG β-galactosidase activity in 120 nM treated BxPC-3, SBLF-7 and SBLF-4 cells on day 6. f Flow cytometric determination of the amount of Annexin V−/7AAD- (living cells) compared to all others (dead cells) 7 days after 120 nM camptothecin treatment. p-values were determined from a two-tailed unpaired Mann Whitney U test: ** < 0.01, *** < 0.001