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Fig. 1 | BMC Molecular and Cell Biology

Fig. 1

From: The PP1 regulator PPP1R2 coordinately regulates AURKA and PP1 to control centrosome phosphorylation and maintain central spindle architecture

Fig. 1

PPP1R2 affects centrosome number through interaction with AURKA and PP1. a-b Schematic of constructs used for transfection and representative transfection. c-i ARPE-19 cells were transfected either singly or in combination with plasmids expressing PPP1R2, AURKA, PP1 and empty vector as control (FLAG). Transfected cells were stained for γ-tubulin (green) and α-tubulin (red) and centrosomes were counted in a minimum of 100 cells for each treatment group in three replicates. Insets magnify cellular regions containing centrosomes. Size bar equals 10 μm. j Graphical representation of the frequency of supernumerary centrosomes in cells transfected with each of the indicated plasmids individually or in combination. Statistically significant differences (p < 0.05) between groups are indicated by differing letter notations above the bars and error bars represent standard error of the mean. Statistically significant differences are indicated with asterisks (* = p ≤ 0.01, ** = p ≤ 0.001) compared to control

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