Fig. 2

PPP1R2 interaction with both PP1 and AURKA affects centrosome number and is phosphorylation dependent. a Schematics of the PPP1R2 mutants used for transfection. The left schematic shows the position of phosphorylation site mutants involving the Thr73 residue including both the threonine to alanine phosphonull mutation PPP1R2A (R2A) and threonine to glutamic acid phosphomimetic mutation PPP1R2E (R2E). The right schematic indicates position of PPP1R2 truncations which included PPP1R2 plasmids truncated at either the N-terminus PPP1R2ΔN (R2ΔN), deleting the PP1 binding site, or the C-terminus PPP1R2ΔC (R2ΔC), removing the AURKA binding site. These mutants were tested for their affect on centrosome number (c-f). Cells were transfected with the indicated proteins, fixed, and stained with anti-γ-tubulin (green) and anti-α-tubulin (red). Size bar equals 10 μm. Insets show the region containing centrosomes. g Quantitation of the percentage of cells with supernumerary centrosomes after transfection with the indicated constructs. Statistically significant differences among groups are indicated by differing letter notations above the bars at p ≤ 0.05 (*p ≤ 0.01, **p ≤ 0.001)