Fig. 3

PPP1R2 overexpression increased cell ploidy and accumulation of cells with enlarged nuclei. a Cells were transfected with the indicated FLAG-tagged fusion proteins, fixed and stained for α-tubulin to detect microtubules (red), and DAPI to stain the nucleus (blue). Size bars equal 10 μm. b Quantitation of DAPI intensity after transfection with the indicated constructs and normalized to the control. c Quantitation of cells with increased ploidy after transfection with PPP1R2. Statistically significant differences (p ≤ 0.05) between experimental groups and empty vector control are indicated by differing letters above the bars and asterisks indicate *p ≤ 0.01 and **p ≤ 0.001