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Fig. 4 | BMC Molecular and Cell Biology

Fig. 4

From: The PP1 regulator PPP1R2 coordinately regulates AURKA and PP1 to control centrosome phosphorylation and maintain central spindle architecture

Fig. 4

PP1, PPP1R2, pR2, and AURKA are localized at the midbody. a ARPE-19 cells were fixed and stained for the indicated endogenous proteins (green) localized relative to α-tubulin of the central spindle (red). b Average intensity from the green channel along the central spindle was measured in 10–0.87 μm2 square regions spanning the length of the central spindle in 30 dividing cells and is plotted in (c). Size bar equals 5 μm. Asterisks indicate statistical significance compared to control (*p ≤ 0.001, **p ≤ 0.001) and error bars represent standard error of the mean

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