Fig. 5

PPP1R2 targets PP1 to the midbody. a-f ARPE-19 cells were transfected, fixed, and labeled for PP1 (green) to determine the localization of endogenous PP1 along the central spindle (red) following overexpression of PPP1R2 mutants: a phosphonull Thr73 mutant PPP1R2A (R2A), a phosphomimetic Thr73 mutant PPP1R2E (R2E), a C-terminal truncation PPP1R2ΔC (R2ΔC), and an N-terminal truncation PPP1R2ΔN (R2ΔN). Average intensity from the green channel was measured along the central spindle in equal sized squares (n = 30 dividing cells) and is plotted in (g). The area under the curve was calculated for total PP1 localization intensity throughout the midbody’s structure (h). Error bars represent standard error of the mean. Statistically significant differences (p ≤ 0.05) between experimental groups and empty vector control are indicated by differing letters above the bars and asterisks indicate *p ≤ 0.01 and **p ≤ 0.001