Fig. 8

The effect of PPP1R2 on PP1 and AURKA activity at the centrosome was dependent on its C-terminus. a A graphical representation of the PP1 and AURKA activity of cells expressing each construct is shown. b Protein expression and phosphorylation levels of proteins indicated by the table below the graph were measured using ELISA. Cells were transfected with the indicated constructs, fixed, and labeled for γ-tubulin (red) and either (c) phosphorylated PP1 (pPP1, green) or (d) phosphorylated AURKA (pAURKA, green) antibodies. pPP1 and pAURKA levels at the centrosome were quantified using Metamorph software and are displayed as graphs in C and D. Statistically significant differences (p ≤ 0.05) between experimental groups and empty vector control are indicated by differing letters above the bars and asterisks indicate *p ≤ 0.01 and **p ≤ 0.001