Fig. 2

Immunofluorescent specificity of POPDC mAbs. COS-7 cells were transfected with either (a) human POPDC1 (GFP tag) or (b) human POPDC2 (FLAG tag) and processed as described in “methods”. a mAb POPDC1 1C12 colocalised with transfected POPDC1 and (b) mAb POPDC2 12G12 colocalised with transfected POPDC2. The POPDC1 mAb did not recognise transfected POPDC2 and the POPDC2 mAb did not recognise transfected POPDC1 (not shown). c mAb POPDC1 1C12 localised to the sarcolemma of human skeletal muscle sections. This localisation was prevented by pre-incubation of the mAb with recombinant POPDC1 but not when the mAb was pre-incubated with recombinant POPDC2. Also, d localisation of mAb POPDC2 12G12 to the sarcolemma was not inhibited by pre-incubation with recombinant POPDC1 but this localisation was inhibited when the mAb was pre-incubated with recombinant POPDC2