Skip to main content
Fig. 4 | BMC Molecular and Cell Biology

Fig. 4

From: Biochemical and genetic analysis of Ecm14, a conserved fungal pseudopeptidase

Fig. 4

The prodomain of Ecm14 can be cleaved by chymotrypsin and trypsin enzymes. a Media containing proEcm14-His6 secreted from baculovirus infected Sf9 cells was digested with chymotrypsin or trypsin for the indicated times and analyzed by Western blotting with an anti-His6 antibody. b Following this digest, the resulting mature protein was purified by metal-affinity chromatography and N-terminally sequenced by Edman degradation. Results of the first round of sequencing are shown. c Sites of cleavage, as shown by Edman degradation, within the region of Ecm14 linking the prodomain and the carboxypeptidase-like domain, are indicated by arrows

Back to article page