Table 1 Analysis of isolated synthetic lethals
From: Biochemical and genetic analysis of Ecm14, a conserved fungal pseudopeptidase
Name | pSLS1(−) rescue (% red colonies) | pSLS-ECM14 rescue (% red colonies) | # of causative mutations | Possible causative mutationa |
|---|---|---|---|---|
WT | 0 | 0 | – | – |
SL1 | 3.9 | 2.7 | 3 | ND |
SL2 | 0 | 0 | ≥4 | ND |
SL4 | 0 | 0 | ≥4 | ND |
SL6 | 16.8 (1/6) | 20.6 (1/5) | 1 | SHM2 D219N (ade3 SL) |
SL7 | 21.0 (1/5) | 18.5 (1/5) | 1 | SHM2 D219N (ade3 SL) |
SL8 | 7.7 | 0 | 3 | Â |
SL9 | 0 | 1.5 | ≥4 | ND |
SL10 | 0 | 0 | ≥4 | ND |
SL11 | 10.5 (1/10) | 10.6 (1/10) | 2 | SHM2 G403R (ade3 SL) |
SL12 | 0 | 0 | ≥4 |  |
SL13 | 0 | 0.8 | ≥4 | SHM1 P252S (ade3 SL) |
SL14 | 6.9 | 8.9 | 2 | SHM2 G252D (ade3 SL) |
SL15 | 15.0 | 9.1 | 2 | ND |
SL18 | 0 | 0 | ≥4 | ND |
SL20 | 5.0 | 0 | 3 | ND |
SL21 | 15.1 | 7.6 | 2 | ND |