Fig. 6

Expression of scavenger receptors and immune lectins in rat LSECs and KCs. a. Unscaled heatmaps of normalized log₂ expression values (log₂ (RPKM+ 1), and log₂ (iBAQ+ 1)) for scavenger receptors (SR) and C-type lectins in the KC and LSEC transcriptomes and proteomes. Underlined: Genes expressed in the transcriptome that were also present in the proteome. b. Absolute abundance of selected SR gene products in the KC and LSEC transcriptomes and proteomes. The bar height reflects good correlation between the transcriptome and proteome data for gene products of Clec4g, Clec4m, Stab1, and Stab2 in both cell types. The abundance of gene products of Marco and Cd5l were well correlated between the KC transcriptome and proteome, while LSECs showed high abundance of these gene products only at mRNA level. c. Immune labeling of non-parenchymal liver cell (NPC) cultures for selected SRs and C-type lectins. NPCs from the 25–45% interface on the Percoll gradient were incubated for 1 h, then fixed 15 min in 4% paraformaldehyde, and double immune-labeled with antibodies to FcγRIIb2 (SE-1; red fluorescence; left column), or CD68 (red fluorescence; right column), and to either stabilin-2 (STAB2; green), mannose receptor (MRC1; green), SR-A1 (green), or SR-B1 (green). Overlap of green and red fluorescence is seen as yellow staining in the overlay images. Antibodies are listed in Table 1. Cell nuclei were stained with DAPI (blue). Arrow heads point to CD68 positive KCs. Antibodies to stabilin-2 and FcγRIIb2 (SE-1) specifically labeled LSECs and the CD68-antibody specifically labeled KCs, whereas positive labeling for the mannose receptor, SR-A1, and SR-B1 was observed in both LSECs and KCs