Fig. 2

Detection of C. elegans transcription by PCR and gel electrophoresis. a Scheme of gel-based detection of C. elegans transcription. A linear DNA containing a transcription promoter was used as the template. Nuclear extract was added into the reaction to synthesize RNA, followed by RNA purification and reverse transcription. The resulting cDNA was amplified by PCR. The DNA product was then run on agarose gel, stained with SYBR Safe Stain, and imaged with a CCD camera. b and c Gel images of the products of transcription reactions with HNDNA b or with PESNDA c. Lanes 1 and 2, transcription with C. elegans nuclear extract; lane 3, transcription with HeLa nuclear extract; lane 4, transcription negative control (without any nuclear extract); lane 5, PCR negative control (without any nucleotides); lane 6, PCR positive control