Fig. 4

Inhibition of in vitro C. elgeans transcription. a α-amanitin was added into transcription reactions containing C. elegans nuclear extract and PESDNA, followed by qRT-PCR quantification. The RNA copy numbers were normalized against reactions with 0 μM α-amanitin. The graph represents the combined results of three independent experiments. b Nuclear extract prepared from ama-1 RNAi wroms or control worms was used for transcription reactions, followed by qRT-PCR quantification. The graph represents the combined results of three independent experiments. Asterisk (*) denotes a significant difference (P < 0.05) between ama-1 RNAi and the control, as analyzed by a two-sample t test for independent samples