Fig. 4

The identification of the key regions in the CD4 gene that led to the functional difference between haplotypes A and B in pigs. a The different chimeras of haplotypes A and B of the CD4 gene. Haplotypes A (blue) and B (red) were divided into three clusters that covered 1st–7th, 8th–13th, and 14th–22nd SNPs. AAB and BBA represented for the mutation of 14th–22nd SNPs of haplotypes A and B. ABB and BAA represented for the mutation of 1st–7th SNPs. ABA and BAB represented for the mutation of 8th–13th SNPs. b Confocal images of the immune stain of the GFP (green) protein. Nucleus was stained with DAPI (blue). Scale bars: 10 μm. c Western blotting results of the CD4-GFP fusion protein at 24 h and 48 h post-transfection using GFP antibody. β-actin was used as internal control