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Fig. 2 | BMC Molecular and Cell Biology

Fig. 2

From: Regulation of the Fasciola hepatica newly excysted juvenile cathepsin L3 (FhCL3) by its propeptide: a proposed ‘clamp-like’ mechanism of binding and inhibition

Fig. 2

Immunolocalisation of FhCL3 zymogen and FhCL3 propeptide within F. hepatica NEJs. NEJs cultured for 6 h and 24 h post-excystment were fixed with 4% paraformaldehyde, washed and then probed with pre-immune rabbit antiserum, anti-FhCL3 zymogen polyclonal antibodies raised in rabbit, or anti-ppFhCL3 polyclonal antibodies raised in rabbit. Immunolocalisation of proteins was visualised using a secondary antibody fluorescein isothiocyanate (FITC)-labelled goat anti-rabbit IgG (green fluorescence). Counter-staining with phalloidin-tetramethylrhodamine isothiocyanate (TRITC) provided the background structure (red fluorescence). OS: oral sucker; VS: ventral sucker. Scale bars, 20 μM

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BMC Molecular and Cell Biology

ISSN: 2661-8850

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