Table 3 Assay conditions of each cysteine peptidase screened against the wild-type ppFhCL3, ppFhCL3 variants and synthetic peptide
Enzyme | Substrate |
|---|---|
F. hepatica cathepsin L1 | Z-Leu-Arg-NHMec (20 μM) |
(FhCL1; 2.7 nM) | |
F. hepatica cathepsin L2 | Z-Leu-Arg-NHMec (20 μM) |
(FhCL2; 5 nM) | |
F. hepatica cathepsin L3 | Z-Gly-Pro-Arg-NHMec (20 μM) |
(FhCL3; 5 nM) | |
Human cathepsin L | Z-Phe-Arg-NHMec (20 μM) |
(HsCL; 0.2 nM) | |
Human cathepsin K | Z-Phe-Arg-NHMec (20 μM) |
(HsCK; 2 nM) |