Fig. 1

Identification of novel splicing variants in canine BRCA2 in ovary and testis. a RT-PCR products around the canine BRCA2 intron 1 in two ovary and one testis samples were electrophoresed. Splicing variants, referred to as splicing variant I-VI and registered sequence are indicated. b Diagram of novel splicing variants in canine BRCA2 intron 1. Arrows indicate primers using RT-PCR and nested-PCR. c RT-PCR products from canine cell lines were electrophoresed and splicing variants excluding splicing variant III were detected. RT indicates RT-PCR products and non-RT indicates PCR products without reverse transcription. Arrows indicate primers used in RT-PCR and nested-PCR