Fig. 3

Human cell lines, HeLa, and 293 T cells also expressed splicing variants. a RT-PCR products between human BRCA2 exon 1 and exon 2 in HeLa and 293 T cell lines with or without 48-h serum starvation were electrophoresed. RT indicates RT-PCR products and non-RT indicates PCR products using the templates without reverse transcription. Arrows indicates primers using RT-PCR and nested-PCR. “§” indicates PCR products with first PCR primers. b Diagram of novel splicing variants in human BRCA2 intron 1. Arrows indicate primers used in RT-PCR and nested-PCR. c Relative expression ratio of registered sequence (ordinary exon 1–2) versus total human BRCA2 expression (exon 25–26) in HeLa and 293 T cells was measured via quantitative PCR. To quantify the level of the registered sequence, the exon 1 and 2 spanning primer was used. d Relative expression level of human BRCA2 normalized using human RPS18 in HeLa and 293 T cells. e HeLa cells treated with 10 Gy X-rays were harvested at the indicated time points after irradiation. The relative expression ratio of registered sequence (ordinary exon 1–2) versus total human BRCA2 expression (exon 25–26) was measured as in Fig. 3 (c). “**” indicates p < 0.01. “N. S.” indicates not significant