Fig. 2

Structure determination of recombinant APETx1 at pH 6.0 and 298 K. a Stereo view of the ensemble of the 20 lowest-energy structures of recombinant APETx1, colored as follows: heavy atoms of backbone, navy; non-cysteine side chain, magenta; cysteine side chain, orange. b Stereo view of the overlay of the 20 structures of recombinant APETx1 at pH 6.0 and 298 K from the present study (PDB code: 7BWI; navy) and the 25 structures of the natural product at pH 3.0 and 280 K from a previous study (PDB code: 1WQK; red) [29]