TY - JOUR AU - Zhang, Xing AU - Yang, Hongwei AU - Jia, Yingdong AU - Xu, Zhengwen AU - Zhang, Liuping AU - Sun, Meng AU - Fu, Jing PY - 2021 DA - 2021/01/20 TI - circRNA_0005529 facilitates growth and metastasis of gastric cancer via regulating miR-527/Sp1 axis JO - BMC Molecular and Cell Biology SP - 6 VL - 22 IS - 1 AB - Circular RNAs (circRNAs) are endogenous non-coding RNAs, which are associated with various biological processes, including microRNA (miRNA) interaction, protein binding and regulatory splicing. circRNA_0005529 (circ_0005529) is derived from vacuolar protein sorting 33 homologue B (VPS33B), and its biological role in gastric cancer (GC) has not been examined. In this study, the expression and location of circ_0005529 and microRNA-527 (miR-527) were determined by qRT-PCR and fluorescence in situ hybridization (FISH). Cell proliferation and cell migration were determined by MTT, EdU incorporation, colony formation, wound scratch and transwell assays. In addition, immunohistochemistry and western blotting were performed to determine the expressions of specificity protein 1 (Sp1), PCNA, c-myc, E-cadherin and N-cadherin. Western blotting and luciferase reporter assay were performed to study the interaction between circ_0005529 and miR-527 or miR-527 and Sp1. The functional effects of circ_0005529 on GC through regulating Sp1 were further evaluated using xenograft and metastatic mouse models in vivo. SN - 2661-8850 UR - https://doi.org/10.1186/s12860-020-00340-8 DO - 10.1186/s12860-020-00340-8 ID - Zhang2021 ER -