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Fig. 4 | BMC Molecular and Cell Biology

Fig. 4

From: circRNA_0005529 facilitates growth and metastasis of gastric cancer via regulating miR-527/Sp1 axis

Fig. 4

circ_0005529 could sponge and negatively regulate miR-527 expression. a Relative luciferase activity of the circ_0005529 reporter plasmid in MKN-45 cells upon transfection of miR-1251, miR-1257, miR-224, miR-518a, miR-527, miR-671-5p and miR-873. b RNA fluorescence in situ hybri-disation for circ_0005529 and miR-527 was performed in HGC-27 and MKN-45 cells (green, circ_0005529; red, miR-527; blue, DAPI). c Relative miR-527 levels in HGC-27 cells transfected with NC inh or miR-527 inh and in MKN-45 cells transfected with NC mimics or miR-527 mimics, as determined using qRT-PCR. d Seeds match for miR-527 in the sequence of circ_0005529. The predicted seed-recognition site in the circ_0005529 sequence and the corresponding miR-527 sequence are marked in green. e Relative luciferase activity of the circ_0005529 reporter plasmid in HGC-27 cells upon miR-527 inh or NC inh transfection and MKN-45 cells upon miR-527 mimics or NC mimics transfection. The mutant circ_0005529 reporter was used as a negative control. f Relative expression levels of miR-527 in HGC-27 cells (Lv-NC or Lv-circ_0005529) and MKN-45 cells (sh-NC or sh-circ_0005529), as determined using qRT-PCR. g Relative miR-527 levels in GC tissues (tumor, n = 63) and normal adjacent tissues (adjacent, n = 63), as determined using qRT-PCR. (H) Pearson’s correlation analysis of the relative expressions between miR-527 and circ_0005529 in GC patients. (Mean ± SEM, * p < 0.05, ** p < 0.01)

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