Fig. 6

circ_0005529/miR-527 promoted the proliferation and migration of GC cells through regulating Sp1 expression. a Growth curves of MKN-45 cells (sh-NC+Control, sh-circ_0005529+Control, sh-circ_0005529+Sp1) at 0 h, 24 h, 48 h, 72 h and 96 h. Measurements of the cell growth rate were obtained using a MTT cell proliferation assay kit. b Protein expression levels of Sp1, PCNA, c-myc, E-cadherin and N-cadherin in MKN-45 cells (sh-NC+Control, sh-circ_0005529+Control, sh-circ_0005529+Sp1), as determined using western blotting. Bands were quantified and shown in histogram. c Representative profiles of EDU cell growth in MKN-45 cells (sh-NC+Control, sh-circ_0005529+Control, sh-circ_0005529+Sp1). EDU incorporation rate were quantified. d Colony formation analysis of MKN-45 cells (sh-NC+Control, sh-circ_0005529+Control, sh-circ_0005529+Sp1). Percentage of colony area were quantified. e Wound scratch healing assay of MKN-45 cells (sh-NC+Control, sh-circ_0005529+Control, sh-circ_0005529+Sp1). Quantification of the wound-healing assay was shown as histograms. f Representative migration and invasion assay images of MKN-45 cells (sh-NC+Control, sh-circ_0005529+Control, sh-circ_0005529+Sp1). The migrated and invaded cells were quantified and shown as histograms. (Mean ± SEM, * p < 0.05, ** p < 0.01)