Fig. 7

Knockdown of circ_0005529 inhibited GC growth and lung metastasis in vivo. a Image of corresponding tumors dissected from mice 21 days post-implantation. Volumes and weight of the xenograft tumors derived from subcutaneous implantation of MKN-45 cells (sh-NC or sh-circRNA). b IHC staining of Sp1, Ki67 and E-cadherin in the xenograft tumors derived from subcutaneous implantation of MKN-45 cells (sh-NC or sh-circRNA). Scale bar = 50 μm. c Tumor metastasis progression was measured by in vivo luciferase imaging at days 30 after intravenous injection of MKN-45 cells (sh-NC or sh-circRNA). d Representative images and H&E staining analysis of lungs dissected from mice with intravenous injection of MKN-45 cells (sh-NC or sh-circRNA). (Mean ± SEM, * p < 0.05, ** p < 0.01)