Fig. 10

CvDCX1 cannot rescue the structural and lytic cycle defects of the TgDCX knockout parasite. a-c EM images of the conoid region of negatively stained T. gondii. Parental RHΔku80Δhx (a, “WT”), TgDCX knockout (b, “ΔTgDCX”, two examples); a clone of TgDCX knockout parasite stably expressing CvDCX1-mNeonGreenFP under control of the T. gondii α-tubulin promoter (c, “ΔTgDCX/CvDCX1”, three examples). Compare with Fig. 6g and h. d Plaque assay (see Methods). Knockout parasites complemented with TgDCX-eGFP expressed under control of the T. gondii α-tubulin promoter (“ΔTgDCX/TgDCX”). Other annotations same as in a-c. Compare with Fig. 6i