A doublecortin-domain protein of Toxoplasma and its orthologues bind to and modify the structure and organization of tubulin polymers

Table 1 Sequence conservation and microtubule interaction properties among TgDCX and its orthologues

orthologue	DCX domain	P25α-DCX domain	P25α to DCX¹	N-term. extension²	T. gondii location³	Xenopus⁴
(size, aa)	(identical/similar, %)	(identical/similar, %)	(aa)	(aa)
TgDCX (256)	(100/100)	(100/100)	44	62	A,D,T	B,B,B
CvDCX1 (213)	46/65	50/60	44	31	A,D,T	B,B
CvDCX2 (174)	33/54	36/52	38	4	a,D,C
CvDCX3 (175)	32/53	35/52	38	3	a,D,C
VbDCX1 (190)	48/71	47/63	39	14	a,D,c⁵
VbDCX2 (168)	39/56	39/53	45	8	a,D,C
VbDCX3 (184)	39/56	41/56	45	0	a,D,C
PfDCX (238)	45/71	36/56	35	83	a,D,C	B,B
TaDCX (178)	34/53	36/51	38	7	C	b

1: Length of linker between the P25α domain and the DCX domain
2: Length of sequence preceding the P25α domain
3: Localization in Toxoplasma of the protein when expressed under control of the α-tubulin promoter is specified as: A, adult (mother) conoid strongly; a, adult conoid weakly; D, daughter conoid; T, daughter cortical MT; C or c, bright or dim diffuse cytoplasmic
4: Localization in Xenopus S3 cells is specified as: B or b, binds to microtubules prominently or with high cytoplasmic background; B,B, binds to and bundles microtubules; B,B,B, binds, bundles, and bends microtubules
5: Also prominent mitochondrial localization

ISSN: 2661-8850