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Table 5 DEGs identified in KEGG pathway: Cholinergic synapse. Differential expression indicates the average log2-fold change in RNA-Seq transcript levels from three independent experiments at each sampling period: D7 vs D0, D13 vs D7, D18 v D13, and D28 v D18. The p-values were ≤ 0.001. Negative log2-fold change corresponds to gene downregulation and the positive values indicate gene activation

From: Global transcriptome profile of the developmental principles of in vitro iPSC-to-motor neuron differentiation

Cholinergic synapse

Gene ID

Description

Differential expression

Time

GNG12

guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-12

−2.18897

D0-D7

CACNA1B

voltage-dependent calcium channel N type alpha-1B

−1.8346

D0-D7

KCNQ3

potassium voltage-gated channel KQT-like subfamily member 3

−2.96129

D0-D7

KCNJ12

potassium inwardly-rectifying channel subfamily J member 12

3.350697

D0-D7

CHRNA3

nicotinic acetylcholine receptor alpha-3

2.793838

D0-D7

CHRNB4

nicotinic acetylcholine receptor beta-4

4.925251

D0-D7

ITPR2

inositol 1,4,5-triphosphate receptor type 2

−3.69982

D0-D7

ITPR3

inositol 1,4,5-triphosphate receptor type 3

−2.4065

D0-D7

ADCY2

adenylate cyclase 2

−3.4602539

D0-D7

ADCY6

adenylate cyclase 6

1.892735

D0-D7

CREB5

cyclic AMP-responsive element-binding protein 5

2.033579

D0-D7

FYN

tyrosine-protein kinase Fyn

1.772436

D0-D7

CAMK4

calcium/calmodulin-dependent protein kinase IV

1.419104

D7-D13

ChAT

choline O-acetyltransferase

4.392642

D13-D18

CACNA1B

voltage-dependent calcium channel N type alpha-1B

1.880481

D13-D18

SLC5A7

solute carrier family 5 (high affinity choline transporter), member 7

4.94003258

D13-D18

KCNQ2

potassium voltage-gated channel KQT-like subfamily member 2

3.013059

D13-D18

CHRNA4

nicotinic acetylcholine receptor alpha-4

2.830329

D13-D18

CHRM3

muscarinic acetylcholine receptor M3

3.97881916

D18-D28

ChAT

choline O-acetyltransferase

1.799457

D18-D28

ACHE

Acetylcholinesterase

2.982588

D18-D28

CHRM2

muscarinic acetylcholine receptor M2

3.383752

D18-D28

SLC5A7

solute carrier family 5 (high affinity choline transporter), member 7

2.363264

D18-D28

KCNQ3

potassium voltage-gated channel KQT-like subfamily member 3

3.701006

D18-D28

CHRNA3

nicotinic acetylcholine receptor alpha-3

−2.67044

D18-D28

PRKCB

classical protein kinase C beta type

1.642454

D18-D28

ADCY2

adenylate cyclase 2

3.240853

D18-D28