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Table 6 MN specification based on log2-fold change in RNA-Seq transcript levels at D28 versus D0 of the differentiation protocol. The p-values were calculated with the R-package. Negative log2-fold change corresponds to gene downregulation and the positive values indicate gene activation. ↓ indicates the gene is expected to be downregulated in the MN subtype

From: Global transcriptome profile of the developmental principles of in vitro iPSC-to-motor neuron differentiation

 

Gene Biomarkers

Fold change

p-value

General motor neuron

HB9

4.29740102

0.03478379

ISL1

9.38120075

1.39E-11

ISL2

−2.71940491

0.12115956

LHX3

5.98518477

1.09E-05

Hypaxial motor column

ETV1

−4.14432026

8.33E-05

HB9

4.29740102

0.03478379

ISL1

9.38120075

1.39E-11

ISL2

−2.71940491

0.12115956

Pre-ganglionic column

SMAD1

1.61250783

3.71E-10

NOS1

−1.1688341

0.1409026

ZEB2

4.94457944

9.71E-19

FOXP1

0.76366376

0.10008361

Spinal accessory column

ALCAM

0.82274029

0.14709124

ISL1

9.38120075

1.39E-11

RUNX1

4.90888056

1.01E-20

PHOX2B

9.18779756

1.30E-06

Medial LMC

↓ HB9

4.29740102

0.03478379

ISL2

−2.71940491

0.12115956

FOXP2

5.83531355

9.82E-56

ALDH1A2

5.96267067

8.55E-13

Lateral LMC

↓ ISL1

9.38120075

1.39E-11

ISL2

−2.71940491

0.12115956

FOXP2

5.83531355

9.82E-56

ALDH1A2

5.96267067

8.55E-13

Median motor column

HB9

4.29740102

0.03478379

ISL1

9.38120075

1.39E-11

ISL2

−2.71940491

0.12115956

LHX3

5.98518477

1.09E-05