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Table 1 DIANAmiR-ExTra data: n-3 LCPUFA regulated placental microRNAs with predicted target sites on validated target genes

From: Fetal sex modulates placental microRNA expression, potential microRNA-mRNA interactions, and levels of amino acid transporter expression and substrates: INFAT study subpopulation analysis of n-3 LCPUFA intervention during pregnancy and associations with offspring body composition

MicroRNA

Fold change

Median Cq

P

Genes over threshold

hsa-miR-888

−11.45

34.0

7.29 E-05

33

hsa-miR-375

−6.77

25.1

2.12 E-03

4

hsa-miR-586

−4.50

32.0

6.45 E-05

1

hsa-miR-130b

−2.13

21.5

4.66 E-02

22

hsa-miR-320

−1.40

16.9

2.16 E-03

13

hsa-miR-21

−1.36

15.2

9.12 E-06

11

hsa-miR-522

−1.32

15.6

8.72 E-04

10

hsa-miR-30d

1.29

16.6

5.38 E-03

47

hsa-miR-451

1.30

15.2

2.61 E-02

0

hsa-miR-495

1.35

17.8

1.22 E-12

63

hsa-miR-517b

1.41

17.1

5.16 E-04

43

hsa-miR-139-5p

1.51

18.8

7.22 E-03

13

hsa-miR-99a

1.53

17.5

1.00 E-02

16

hsa-miR-100

1.55

15.5

4.40 E-03

15

hsa-miR-668

3.14

26.0

1.73 E-04

6

hsa-miR-641

3.39

30.9

1.68 E-03

8

hsa-miR-302b

21.22

37.4

3.32 E-03

18

hsa-miR-367

Con (+) / N3 (−)

34.0†

7.80 E-05

23

hsa-miR-649

Con (+) / N3 (−)

33.6†

8.78 E-03

2

hsa-miR-302a

Con (+) / N3 (−)

36.4†

1.47 E-02

21

hsa-miR-569

Con (−) / N3 (+)

35.6†

1.57 E-03

2

hsa-miR-630

Con (−) / N3 (+)

32.5†

2.47 E-02

27

hsa-miR-155

Con (−) / N3 (+)

32.1†

4.65 E-02

10

  1. Using DIANAmiR-ExTra 23 n-3 LCPUFA-regulated placental microRNAs with potential target sites on validated differentially expressed placental target genes following n-3 LCPUFA supplementation (N3) were found. Differentially expressed genes were identified by our previous mRNA microarray analyses (n-3 LCPUFA data sets) [10]. n-3 LCPUFA-regulated placental microRNAs with respective median Cq-values and expression fold changes compared to controls (Con) are listed. Number of significantly n-3 LCPUFA regulated genes with predicted potential binding-sites over threshold for the respective microRNAs by DIANAmiR-ExTra are depicted. P-value calculated by one-sided Wilcoxon rank sum test; microRNAs selected for further validation are marked in bold. Cq, quantification cycle; microRNA expressed in one group (+) but not in the other group (−); †, median Cq not calculated