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Table 3 Data on validated expression of microRNAs, mRNAs, and protein

From: Fetal sex modulates placental microRNA expression, potential microRNA-mRNA interactions, and levels of amino acid transporter expression and substrates: INFAT study subpopulation analysis of n-3 LCPUFA intervention during pregnancy and associations with offspring body composition

 

Control

Intervention

Control

Intervention

P-values regression

 

(Con-F + Con-M)

(N3-F + N3-M)

Con-F

Con-M

N3-F

N3-M

Treatment

Sex

Interaction

Con-M

vs

Con-F

N3-M

vs

N3-F

N3-F

vs

Con-F

N3-M vs

Con-M

 

N

MRE

N

MRE

N

MRE

N

MRE

N

MRE

N

MRE

  

%

± SD

 

%

± SD

 

%

± SD

 

%

± SD

 

%

± SD

 

%

± SD

       

microRNA

miR-30d

16

100 ± 30

19

97 ± 33

9

100 ± 35

7

97 ± 29

10

94 ± 38

9

98 ± 31

0.620

0.756

0.652

0.899

0.596

0.506

0.997

miR-320

18

100 ± 64

19

108 ± 61

10

100 ± 70

8

92 ± 59

10

111 ± 68

9

95 ± 57

0.415

0.515

0.650

0.903

0.438

0.369

0.831

miR-100

19

100 ± 34

18

107 ± 40

11

100 ± 33

8

108 ±37

9

115 ± 41

9

107 ± 42

0.558

0.917

0.389

0.594

0.492

0.313

0.812

miR-99a

19

100 ± 51

19

149 ± 70

11

100 ± 41

8

142 ± 63

10

186 ± 70

9

164 ± 79

0.001

0.511

0.026

0.039

0.266

< 0.001

0.452

mRNA

mTOR

20

100 ± 22

21

122 ± 34

11

100 ± 13

9

119 ± 21

10

136 ± 38

11

129 ± 28

0.008

0.330

0.098

0.062

0.649

0.003

0.441

SLC7A5

20

100 ± 71

21

122 ± 70

11

100 ± 60

9

183 ± 104

10

165 ± 80

11

170 ± 90

0.052

0.019

0.012

0.001

0.955

0.002

0.704

SLC6A6

20

100 ± 60

20

53 ± 25

11

100 ± 40

9

109 ± 67

9

57 ± 22

11

54 ± 18

< 0.001

0.945

0.807

0.825

0.900

< 0.001

< 0.001

Protein

 LAT1

8

100 ± 25

10

77 ± 17

4

100 ± 25

4

71 ± 17

5

74 ± 21

5

58 ± 16

0.010

0.004

0.341

0.010

0.085

0.014

0.183

  1. Validation data of selected placental microRNAs from microRNA profiling and of target mRNAs by qPCR (see respective scatter plots Additional file 3: Figure S1 and S2) and of placental LAT1 (normalized to GAPDH protein, see Fig. 3 and Additional file 3: Figure S3) by western blot are shown. The expression level of the female control offspring (Con-F) samples was assigned an arbitrary value of 100% and all other analyzed groups (Con-M, male offspring of the control group; N3-F and N3-M, female and male offspring of the intervention group, respectively) were calculated relative to Con-F. Data are presented as mean relative expression (MRE) in % + standard deviation (SD). The P-values for Treatment and Sex come from linear regression models fit to the expression data without a Sex-Group Interaction term. The P-values for the Interaction and pairwise group comparisons (Con-M vs Con-F, N3-M vs N3-F, N3-F vs Con-F, N3-M vs CM) are from regression models including a Sex-Group Interaction term. P-values p < 0.05 were considered as significant and are shown in bold