Fetal sex modulates placental microRNA expression, potential microRNA-mRNA interactions, and levels of amino acid transporter expression and substrates: INFAT study subpopulation analysis of n-3 LCPUFA intervention during pregnancy and associations with offspring body composition

Table 4 Correlations among regulated levels of placental microRNA, mRNA, and protein expression

		*miR-99a*	*mTOR*	*SLC7A5*	LAT1
*mTOR*	Rs	*0.35*
	P	0.033
	N	38
*SLC7A5*	Rs	0.43	0.46
	P	0.006	0.003
	N	38	41
LAT1	Rs	−0.39	−0.40	−0.09
	P	0.131	0.101	0.735
	N	16	18	18
*SLC6A6*	Rs	−0.42	− 0.34	− 0.11	0.59
	P	0.009	0.033	0.049	0.010
	N	37	40	40	18

The association analyses were conducted independent of n-3 LCPUFA intervention status. ΔCq-values for mRNA and microRNA expression of respective genes were used; optical density values for LAT1 protein expression normalized to respective GAPDH protein from western blot experiments were used. Positive or negative signs indicate a positive or inverse association, respectively. Rs-values with P-values p < 0.05 are shown in bold. N, number of analyzed placentas; P, p-value for the respective correlation; Rs, Spearman-rho correlation coefficient