Skip to main content
Fig. 1 | BMC Molecular and Cell Biology

Fig. 1

From: The negative charge of the 343 site is essential for maintaining physiological functions of CXCR4

Fig. 1

Schematic diagram of mutations in CXCR4. a 9 different mutations in the C-terminus of CXCR4 cause WHIM syndrome. b The protein sequence of mutant CXCR4. c The glutamic acid at 343 site of CXCR4 is highly conserved among species. d the mutant CXCR4 were generated by mutagenesis and the vector was confirmed by sequencing. e The stably overexpressing wildtype and mutant CXCR4 in HeLa cells were validated by western blot. f Flow cytometric analysis of GFP expression in control (negative control, NC) Hela cells and HeLa cells stably transduced with CXCR4 (WT, E343D, E343K, E343R and E343A)

Back to article page