Fig. 4

Luciferase activity assay after overexpression of miRNA precursors. HEK393T cells were co-transfected with recombinant plasmids, which included MSI1 3’UTR downstream humanized Renilla luciferase reporter gene (hRluc) and miRNAs precursors. Also, HEK393T cells co-transfected with mutated Msi1 3’UTR for each miRNAs binding site downstream of hRluc luciferase reporter gene. For negative control analysis, HEK393Tcells co-transfected with a scrambled negative control miRNA (Control miRNA) and wild Msi1 3’UTR downstream hRluc. Then luminescence was measured. Error bars denote S.E.M. for three independent repeats in each experiment. Independent student t-test indicated differences between (*p < 0.05; **p < 0.01)